Overview. XLD Agar (Xylose Lysine Deoxycholate Agar) is a moderately selective differential medium used for the isolation and differentiation of enteric gram-negative pathogens, particularly salmonella and shigella in clinical, environmental and food samples. It is based on the fermentation of xylose, lactose and sucrose, the decarboxylation of.. The key ingredients of XLD agar are three sugars (xylose, lactose, and sucrose), lysine, and ferric ammonium citrate. Yeast extract provides sources of nitrogen, carbon, and vitamins required for organism growth. Sodium deoxycholate present in the medium inhibits the growth of Gram-positive organisms. Sodium chloride maintains the osmotic.
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Xylose Lysine Deoxycholate (XLD) Agar is a selective medium for the isolation of Salmonella and Shigella spp from clinical specimens and food samples.XLD Agar was originally formulated by Taylor for the isolation and identification of Shigella from stool specimens.. The pathogens are differentiated not only from the non-pathogenic lactose fermenters but also from many non-pathogens which do.. This chapter highlights that xylose lysine deoxycholate (XLD) agar is developed for the isolation of salmonellae and shigellae. It relies on the use of the selective inhibitory component sodium deoxycholate and three indicator systems—namely, (1) xylose, lactose, sucrose combined with phenol red,(2) lysine HCl and phenol red, and (3) sodium thiosulfate and iron.
